HIGH YIELD POINT FOR MICROBIOLOGY 2015

HIGH YIELD POINT FOR MICROBIOLOGY 2015

Burkholderia pseudomallei is the causative agent of melioidosis. It also causes chronic pulmonary infection mimicking TUBERCULOSIS. The other principal form of B. pseudomallei infection is skin ulceration with associated lymphangitis and regional lyphadenopathy. Spread from the lungs or skin, which is most often documented in debilitated individuals give rise to septicemic forms of meliodosis that carry a high mortality rate. H1N1 influenza–> incubation period–> 3-7 days.

Prompt initiation of antiviral agents (oseltamivir or zanamivir) within 48 hours of symptom onset is imperative for providing treatment efficacy against influenza virus.

Lambda phage will remain in lysogenic state if cl proteins predominate, but will be transformed into the lytic

cycle if cro proteins predominate

Prion- In mammals prions reproduce by binding to normal, cellular isoform of the prion

protein(PrPC) and stimulating conversion of PrPC into the disease causing isoform(PrPSc). PrPC is rich in alpha-helix and has little beta-structure, while PrPSc has less alpha-helix and a high amount of beta-structure. This alpha-to-beta structural transition in the prion protein(PrP) is the fundamental event underlying prion diseases. All known prions induce the formation of an amyloid fold, in which the protein polymerises into an aggregate consisting of tightly packed beta sheets. This altered structure is extremely stable and accumulates in infected tissue, causing cell death and tissue damage. This stability means that prions are resistant to denaturation by chemical and physical agents, making disposal and containment of these particles difficult.

Interferon are proteins and are the first cytokine to be recognized

Malachite green inhibits gram positive and negative organisms and has no activity against tubercle bacilli. Hence it is used as a selective agent in LJ medium.

SOME IMPORTANT POINTS ABOUT AUTOCLAVE & HOT AIR OVENĀ 

All have equal effect
Autoclaving 121 C x 15 min (M/C)
126 C x 10 min
134 C x 3 min
Hot air oven 170 C x 18 min
180 C x 7.5 min
190 C x 1.5 min